The fermentation process development groups have experience with both yeast (K. lactis and S. cerevisiae) and E. coli expression systems. Titers of 2-3 g/L soluble proteins have been routinely achieved using an E. coli inducible expression system and chemically defined media. We are involved From Gene to Commercial Manufacturing, and can optimize and adapt processes from initial gene sequence to final harvest procedures
- Strain construction
- RCB
- GMP Cell Banking (MCB, WCB, EOP)
- Tech Transfer
- Process Development
- Process optimization
- Scale-Up
- Transfer to Manufacturing
- Manufacturing Support
- Process risk assessment
- Down-Scaling
- Process characterization
The main focus for the fermentation process development is to develop a process that is robust and scalable, with high biomass, good specific productivity and high final product titer, using a regulatory friendly medium.