Mammalian Cell Line Development
CMC Biologics’ Mammalian Cell Line Development platform integrates CHEF1® high productivity expression plasmids, superior performance serum-free suspension adapted (SFSA) CHO cells and custom production media. All three components of this integrated platform have been co-developed to create optimal growth conditions yielding high cell densities resulting in consistent, stable expression of our clients’ proteins in rapid timeframes.

• Serum free cells and transfection
• CHEF1 expression plasmids
• Serum-free cloning and monoclonal documentation by  microscopic imaging
• FACS screening: Isolation of stable, high-productivity clones
• Complete development documentation

Serum-free Cells
CMC Biologics has developed a serum-free suspension-adapted (SFSA) CHO cell line with superior cell culture performance.  Our SFSA cells are propagated entirely in serum-free media and adapted to chemically defined CD-CIM1.  High-cell density production growth, in excess of 10 million cells per milliliter, is routinely achieved in production models.  These cells are available to clients as part of our standard cell line development platform.

FACS - Cell Screening
Manufacturing cell line development relies heavily on identification and isolation of high-expressing clones from pools of non-clonal cells.  Our cell screening technologies utilize flow cytometry as part of our standard platform and custom cell line screening projects.  Flow cytometry is a rapid technique for primary screening and isolation of high-expressing clones, thereby expediting the clone selection process.

Custom Services
Molecular Services

• CHEF1 vector construction
• Cell line stability and genetic characterization
• Gene sequence confirmation

Cell Line Services

• Cloning of existing client mammalian cell lines
• Characterization and expression stability of mammalian cell lines

FACS Screening Services

• Enrichment of high producing cells in transfected CHO pool population
• Identification of high producing CHO clones